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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in lots of tumors, which ends up in chromosome instability, and therefore may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical job in promoting cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already explained. Two extra splice variants ended up noted but comprehensive nucleotide sequence information and facts is just not yet readily available.
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Blocking peptides are peptides that bind particularly for the focus on antibody and block antibody binding. These peptide commonly incorporates the epitope recognized by the antibody.
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies bound to the blocking peptide no more bind to the epitope within the goal protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is particular.
Target Information and facts Cyclin E belongs on the highly conserved cyclin loved ones, whose members are characterised by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which add for the temporal coordination of each mitotic celebration. Cyclin E varieties a posh with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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